TABLE 3.

Production of immunoactive IL-1β^a

Transformant	Carbon sourceb	Total amt of IL-1β producedc		Secreted IL-1β (%)
		μg/ml	pg/cell
1	Galactose	101	1.7	87
	Glucose	40	0.15	90
2	Galactose	69	1.0	75
	Glucose	40	0.12	76
3	Galactose	97	0.9	80
	Glucose	45	0.12	78

^aThe amount of immunoactive IL-1β was determined by ELISA on both culture medium and cell extracts. Three independent BT16(pGA-IL) transformants were analyzed after 24 h in production medium (LPi). 

^bCarbon source in the precultivation medium. 

^cTotal amount of IL-1β (supernatant and cell extracts) produced per milliliter or per cell.