Fig. 3.

DYRK1A enhances EMT and the migration and invasion abilities of HCC cells. a and b HCC cells were incubated with the DYRK1A overexpression plasmid or empty vector for 24 h, and cell migration and invasion assays were then carried out. c HCC cells were incubated with the DYRK1A overexpression plasmid or empty vector for 24 h, and a scratch was then created. Following incubation for 24 h, images were acquired under a microscope. d Western blot analysis was performed in the DYRK1A knockdown group and control group. e Following treatment with harmine for 24 h at the indicated concentrations, total protein was extracted from HCC cells and analysed by western blotting. f HCC cells were incubated with the DYRK1A overexpression plasmid or empty vector for 48 h. Subsequently, total protein was extracted from HCC cells and analysed by western blotting. g HCC cells were incubated with DYRK1A siRNA or control siRNA for 48 h, and mRNA levels were determined. h HCC cells were incubated with DYRK1A overexpression plasmid or empty vector for 48 h, and mRNA levels were determined