TABLE II. Primers and TaqMan probes sequences for Trypanosoma cruzi validated quantitative polymerase chain reaction (qPCR).

Molecular target	Methodology	Primer/Probe sequences (5’-3’) or Kit company/Consortium	Reference
T. cruzi satellite DNA	Conventional PCR or real time PCR	TcZ-F “GCTCTTGCCCACAMGGGTGC”	82
		Tcz-R “CCAAGCAGCGGATAGTTCAGG”
	Standardised in-house TaqMan qPCR	Cruzi 1 “ASTCGGCTGATCGTTTTCGA”	82,85
		Cruzi 2 “AATTCCTCCAAGCAGCGGATA”
		Cruzi 3 Probe “FAM-CACACACTGGACACCAA-NFQ-MGB”
	Comercial real time PCR	Wiener Lab-CONICET-ANLIS MALBRAN-Argentina	54
		RealCycler CHAG; Progenie Molecular, Spain	60
		TCRUZI DNA.CE Diagnostic Bioprobes Srl, Italy	61
	Comercial LAMP	Eiken Chemical Company, Japan	53,59
T. cruzi minicircle DNA	Conventional PCR	121 “AAATAATGTACGGGKGAGATGCATGA”	50,51,52,82
		122 “GGTTCGATTGGGGTTGGTGTAATATA”
	Standardised in-house TaqMan qPCR	32F “TTTGGGAGGGGCGTTCA”	85
		148R “ATATTACACCAACCCCAATCGAA”
		71P Probe “FAM-CATCTCACCCGTACATT-BHQ1 a ”
	Comercial real time PCR	Real STAR Chagas, ALTONA Diagnostics, Germany	62

a: C locked nucleic acid (LNA) nucleotides.