Table 4.
Potency of Representative Compounds in Multiple Assays
| Cell proliferation (IC50, nM) | ATP Depletion (IC50, nM) | NAD+/NADH ratio (IC50, nM) | |||
|---|---|---|---|---|---|
|
| |||||
| Glucose medium | Galactose medium | Glucose medium | Galactose medium | Glucose medium | |
| 16 | >3000 | 19.4±3.3 | >3000 | 21.3±2.2 | 23±18.1 |
| 20 | >3000 | 72.4±12.6 | >3000 | 78.9±11.3 | 34±15 |
| 21 | >3000 | 135.1±21 | >3000 | 123.3±8.1 | 67.5±18.8 |
| 23 | >3000 | 9.1±5.9 | >3000 | 11±1.5 | 3.6±3.2 |
| 27 | >3000 | 12.6±8.9 | >3000 | 15.8±2.4 | 3.2±1.3 |
a
IC50 values of proliferation in glucose- and galactose-containing medium were determined using a 3-day MTT assay in MIA PaCa-2 cells.
b
IC50 values of ATP production in glucose- and galactose-containing medium were determined using CellTiter-Glo after 1 day treatment in MIA PaCa-2 cells.
c
IC50 values of each compound on the inhibitory effect of NAD+/NADH ratio was determined by NAD/NADH-Glo™ Assay upon 2 days treatment in MIA PaCa-2 cells.