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. 2022 Feb 16;76(3):521–545. doi: 10.1007/s11418-022-01605-6

Table 2.

Strengths and weaknesses of the LFA for phytoproducts analysis

Strengths Weaknesses
Ready to use High cost and high labor intensive for antibody production
Rapid device preparation time Non-specific binding of antibody possibly occurs
Simple analytical procedure Questioning reproducibility (especially lot-to-lot)
Analyte-specific method Yes/no results output
Shorter analytical time required compared to conventional chromatographic methods and ELISA Comparator for semi-quantitative analysis is required
No sample pretreatment step needed The sensitivity is generally lower than ELISA
Controlled storage condition is not required
Easy to scale-up
Cost-effective method
Easy to convert the signal to value
Applicable in many scenarios
No signal reader needed
No previous sample contamination
Low organic solvent requirement
Low sample volume needed