Table 2.
Strengths and weaknesses of the LFA for phytoproducts analysis
Strengths | Weaknesses |
---|---|
Ready to use | High cost and high labor intensive for antibody production |
Rapid device preparation time | Non-specific binding of antibody possibly occurs |
Simple analytical procedure | Questioning reproducibility (especially lot-to-lot) |
Analyte-specific method | Yes/no results output |
Shorter analytical time required compared to conventional chromatographic methods and ELISA | Comparator for semi-quantitative analysis is required |
No sample pretreatment step needed | The sensitivity is generally lower than ELISA |
Controlled storage condition is not required | |
Easy to scale-up | |
Cost-effective method | |
Easy to convert the signal to value | |
Applicable in many scenarios | |
No signal reader needed | |
No previous sample contamination | |
Low organic solvent requirement | |
Low sample volume needed |