Table 3.
Nanoparticles used in LFA development applied for phytoproducts
Nanoparticles | Color | Detection method | Strength | Weakness |
---|---|---|---|---|
Colloidal gold nanoparticles | Red-pink | Visual observation | Ease of labeling (physical adsorption) | Less color intensity lead to low sensitivity |
Strip reader (quantitative analysis) | Manageable surface | False positive and false negative possibly occur | ||
Photo analysis software (optional) | Widely used | Performance in red-yellow colored samples is low | ||
Commercially available | ||||
Ease of in-house synthesis and functionalization | ||||
Stable in various LFA condition | ||||
Quantum dots | Depend on the material | Fluorescence strip reader | Ease of labeling (chemical conjugation) | Expensive |
Visual observation (optional/lower sensitivity) | Low photo-bleaching | Fluorescent strip reader needed | ||
Stable in various LFA condition | Toxic to the environment | |||
High sensitivity | ||||
Wide range absorption spectra | ||||
Specific emission wavelength | ||||
Photostability | ||||
Colloidal carbon | Black | Visual observation | Ease of labeling (physical adsorption) | Performance in black colored samples is low |
Photo analysis software (optional) | Ease of In-house synthesis | |||
Cost-effective | ||||
Stable in various LFA condition | ||||
Low toxicity | ||||
High contrast results (black and white) |