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. 2022 Feb 16;76(3):521–545. doi: 10.1007/s11418-022-01605-6

Table 3.

Nanoparticles used in LFA development applied for phytoproducts

Nanoparticles Color Detection method Strength Weakness
Colloidal gold nanoparticles Red-pink Visual observation Ease of labeling (physical adsorption) Less color intensity lead to low sensitivity
Strip reader (quantitative analysis) Manageable surface False positive and false negative possibly occur
Photo analysis software (optional) Widely used Performance in red-yellow colored samples is low
Commercially available
Ease of in-house synthesis and functionalization
Stable in various LFA condition
Quantum dots Depend on the material Fluorescence strip reader Ease of labeling (chemical conjugation) Expensive
Visual observation (optional/lower sensitivity) Low photo-bleaching Fluorescent strip reader needed
Stable in various LFA condition Toxic to the environment
High sensitivity
Wide range absorption spectra
Specific emission wavelength
Photostability
Colloidal carbon Black Visual observation Ease of labeling (physical adsorption) Performance in black colored samples is low
Photo analysis software (optional) Ease of In-house synthesis
Cost-effective
Stable in various LFA condition
Low toxicity
High contrast results (black and white)