Figure 3.

IL-17A deficiency alleviates DE-induced cognitive impairment as detected in the novel object recognition test.

(A) The habituation of mice in an open-field environment was performed 1 day before the novel object recognition testing. Representative grid crossing paths are shown, and no significant differences in crossing numbers were observed among the groups. (B) Il17a gene knockout significantly improved discrimination index values relative to those for DE mice. Representative tracking paths for mice in the novel object recognition test are shown. The upper and lower rows show the training and testing tracks, respectively. The IL-17A-KO DE mice spent more time exploring novel objects than the DE mice. Mice were analyzed at 32 weeks of age. Data are expressed as means ± SD (n = 8). *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Student-Newman-Keuls test). DE: Diabetic encephalopathy; IL-17A: interleukin 17A; KO: knockout; N: novel object; O: old object; WT: wild type.