Skip to main content
. 2022 Apr 10;9(16):2103135. doi: 10.1002/advs.202103135

Figure 6.

Figure 6

LINC01431 increases the occupancy of PRMT1 on HBV cccDNA, which promotes H4R3me2a and induces cccDNA silencing. ChIP assays were performed in MC‐HBV‐transfected Huh7 (A,B,E,F), HBV‐infected HepaRGNTCP (C), and HBV‐infected HLCZ01 cells (D). A–D) ChIP assays were performed in Huh7 cells, HepaRGNTCP cells, and HLCZ01 cells with LINC01431 overexpression or knockdown, and the enrichment of PRMT1 and H4R3me2a as well as epigenetic modifications were detected using indicated antibodies. E,F) Rescue assays were performed in MC‐HBV‐transfected and LINC01431 overexpressed Huh7 cells after silencing PRMT1 or in the presence of the PRMT1 inhibitor C‐7280948 (12.8 µm). The enrichment of PRMT1, H4R3me2a, and epigenetic modifications on cccDNA were measured using indicated antibodies. For all experiments, representative of 3 independent experiments. Data information: data were presented as mean ± SD and normalized to the control group. One‐way ANOVA (C,E,F). Two‐tail unpaired Student's t‐tests; *p < 0.05; **p < 0.01; NS, no significance (A,B,D).