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. 2022 Mar 24;9(16):2200050. doi: 10.1002/advs.202200050

Figure 3.

Figure 3

Evaluation tools of OC regeneration. A) Analysis techniques used to assess OC regeneration. The left panel represents the general techniques used, including histology, macroscopic observation (macro. obs.), Immunohistochemistry (IHC), micro‐computed tomography (Micro‐CT), biomechanics, medical imaging (Med. imaging), molecular biology (Mol. biology), and others. The hatched bars indicate the link with the right panels that represent in detail the different types of histological staining performed (top panel) and the different markers of articular cartilage and subchondral bone evidenced by IHC. HE: hematoxylin & eosin; SafO: Safranin O; SafO/FG: Safranin O counterstained with Fast Green; TB: Toluidin Blue; MT: Masson's Trichrome; AB: Alcian Blue; SR: Sirius Red; Col: collagen types I, II, and X; ACAN: Agreccan; OCN: osteocalcin; MMP13: Matrix Metalloproteinase 13. B) Scoring systems. The graph in the center represents the types of scores used in general. The left graph represents the macroscopic scores in detail. The right graph represents the histological scores in detail. The hatched portions represent the modified scores.