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. Author manuscript; available in PMC: 2022 Jun 4.
Published in final edited form as: Methods Mol Biol. 2022;2404:393–407. doi: 10.1007/978-1-0716-1851-6_22

Fig. 8.

Fig. 8

Final library distribution QC. The QCs shown here were done using Bioanalyzer DNA 1000 high sensitivity chips. (a) RibOxi-seq library after 1 round of agarose gel purification (cutting above 220–250 bp). There is still an empty ligation product peak that is at 200 bp. (b) In our experience, a second round of the purification is required to completely remove the 200 bp empty ligations