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. Author manuscript; available in PMC: 2022 Jun 4.
Published in final edited form as: Methods Mol Biol. 2021;2230:303–323. doi: 10.1007/978-1-0716-1028-2_17

Figure 2:

Figure 2:

Molecular assessment of PLR. Immunohistochemistry for PLR-associated proteins MMP13, MMP14, CTSK and TRAP on femurs isolated from 8-week old C57BL6 mice. 7 μm thin paraffin embedded bone sections are is shown (A). Arrows indicate the osteocytes positive for the respective stain. Scale bar is 20 μm. RT-qPCR highlights the lactation mediated induction in PLR-related genes (B). All genes were normalized to 18s RNA as an internal control gene and relative changes in gene expression in the lactating C57/BL6 mice were compared against virgin controls. N=10 mice in each group and *p>0.05, different from the virgin mice, as calculated from the Student’s t-test.