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. Author manuscript; available in PMC: 2022 Jun 4.
Published in final edited form as: Methods Mol Biol. 2021;2230:303–323. doi: 10.1007/978-1-0716-1028-2_17

Table 1:

Reverse transcription master mix

Reagents 1 reaction
5X iScript RT Supermix 4 μl
iScript Reverse Transcriptase 1 μl
RNA template (1 μg) variable
Nuclease free water variable
Total volume 20 μl