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. 2022 May 20;13:886913. doi: 10.3389/fneur.2022.886913

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Copyright © 2022 Ma, Wang, Wang, Jin, Ba, Ji, Du and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagram of this study. (A) Differential genes were obtained from GBM and normal brain tissue. (B) Hub genes were identified by Weighted Gene Co-Expression Network Analysis (WGCNA). (C) Clinicopathologic characteristics of 28 hub genes were analyzed. (D) Lasso and univariate regression were used to identify 3 key genes and the corresponding risk coefficients. (E) ICH and TIMER were used to analyse the clinicopathological characteristics of three key genes. (F) ssGSEA was used to analyse immune infiltration in both groups. (G) Maftools and Genomic Identification of Significant Targets in Cancer algorithm were used to analyse mutations and CNV in both groups. (H) ChAMP was used to analyse methylation in high and low-risk groups.