Skip to main content
. Author manuscript; available in PMC: 2022 Jun 4.
Published in final edited form as: Cell Metab. 2021 Oct 21;33(11):2201–2214.e11. doi: 10.1016/j.cmet.2021.10.001

Fig 2. Identification and characterization of human fibro-adipogenic progenitors.

Fig 2.

A-i, Sorting strategy (CD45−CD31−) identifying CD34+CD56− or CD34−; A-ii, CD34+CD90− and CD34+CD90+ cells; A-iii, CD56+CD82+ cells; A-iv, CD90+ cells; B, Work-flow; C-E, adipogenic, fibrogenic and myogenic differentiation of CD34+CD90−CD56− (C), CD34+CD90+CD56− (D) and CD56+CD82+CD34− (E) populations by IHC; F, % mononuclear cells in human skeletal muscle (n=17, study 2, non-T2DM); G, PDGFRα in FAPs (scalebar 50 μm); H, COL1A1 expressing CD90+CD56− (FAPs), CD56+ (MuSCs) and CD90−CD56− (Unidentified) cells (n=6, study 2, non-T2DM) after 3–9 days in vitro; I, Clonal experiment; J, Colony formation (%) of single sorted FAPs and MuSCs (n=12, study 2, non-T2DM); K, Limiting dilution assay on FAPs and MuSCs (n=3, study 2, non-T2DM), Solid line is non-linear fit; L, MyHC+/Desmin+ myotubes from single sorted MuSCs; M, Perilipin1+/Collagen1+ cells from single sorted FAPs; N, Biological processes in FAPs (n=3, study 2, non-T2DM); O, % of EdU+ MuSCs and FAPs (n=10–16, study 2, non-T2DM); P, EdU+ MuSCs (i) and FAPs (ii) 96h post sort. Cells obtained from of mm. Rectus Abdominus/Gastrocnemius. *p<0.05, **p<0.01 and ***p<0.001.