Fig 4. [Ca²⁺]_i oscillations within different acini.

(A) OGB-1 loaded acinar cells in a tissue slice. Cells indicated with 1–3 belong to the same acinus (the thicker broken line marks the acinus, the thinner broken lines mark boundaries between individual acinar cells). Scale bar 20 μm. (B) The [Ca²⁺]_i activity patterns in three different acinar cells from the same acinus (1–3) and in two cells from two other distinct acini (cells 4 and 5) stimulated with 50 nM ACh. Please note that [Ca²⁺]_i oscillations were similar in shape and synchronized between cells in the same acinus but not between cells from different acini (1–3 vs. 4 vs. 5).