FIG. 5.

Type I methanotroph DGGE analysis. Shown is an ethidium bromide-stained 6.5% polyacrylamide denaturing gradient gel (20 to 70%) showing separation patterns based on sequence difference of the V3 region (approximate E. coli positions 341 to 534) of the 16S rDNA. Lanes 1 and 10, the type I landfill methanotrophic community (250 ng and 1.5 μg, respectively); lane 2, clone T1-08; lane 3, clone T1-01; lane 4, clone T1-14; lane 5, clone T1-09; lane 6, clone T1-02; lane 7, clone T1-04; lane 8, isolate AML-C10; lane 9, isolate AML-D4. Bands A through L are explained in the text.