FIG. 6.

DGGE analysis of cloned DNA extracted from type I community bands A and B (Fig. 5). Shown is an ethidium bromide-stained 6.5% polyacrylamide denaturing gradient gel (20 to 70%) showing separation patterns based on sequence differences in the V3 region (approximate E. coli positions 341 to 534) of the 16S rDNA. See Results for experimental details. Lane 1, clone EB-A1; lane 2, clone EB-A2; lane 3, type I landfill methanotrophic community profile; lane 4, clone EB-B1; lane 5, clone EB-B2; lane 6, clone T1-08; lane 7, clone T1-09.