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. 1999 Nov;65(11):4898–4907. doi: 10.1128/aem.65.11.4898-4907.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 5 — (A) Transposition of Tn1720. Shown are results of agarose gel electrophoresis of pGEM3zf(+) undigested (lane 1) and PstI digested (lane 3) and the Amp^r Tc^r plasmid recovered from E. coli JM109 carrying both pGEM3zf(+) and the Tc^r plasmid from Pseudomonas isolate R171A undigested (lane 2), PstI digested (lane 4), and SunI digested (lane 5). Lane M is HindIII-digested lambda DNA. (B) PCR detection of Tn1720. Shown is the PCR product from isolate R171A undigested (lane 1) and Sun I digested (lane 2). Lane M is 1-kb Ladder Plus.