Figure S4.

GO analysis of unaltered genes in ATAC-Seq and effects of AAV-mediated overexpression of CTCF, Foxa2, and Pdx1 on islet insulin secretion. (a) GO Biological Process enrichment analysis of genes annotated to peaks with their chromatin accessibility unaltered by dietary intervention (|log₂FC (HFD-Chow vs. Chow)| > 0.5 AND NOT Reversible Peaks). (b) Immunoblot of total protein lysates from islets transduced with AAV expressing CTCF, Foxa2, Pdx1, or GFP (as control) for 5 d. (c) Dynamic glucose-stimulated insulin secretion (left) and quantified phase I and phase II insulin levels (right) from islets as treated in b. Islets were isolated from mice fed with HFD for 3 mo. Each islet sample was pooled from at least three animals. Data represent mean ± SD, n = 3 technical replicates; ***, P < 0.001; one-way ANOVA. Data in b and c are representative of two independent experiments. Source data are available for this figure: SourceData FS4.