TABLE 2.
Assignment of the nifH clones from the symbiotic microbial community in the gut of termites to phylogenetic groups
| Termite species | Clone name taga | Number of clones
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Proteo-cyano | Anaerobe | anf-methano | Pseudo nif | Other | Frameshiftc | Total | Different AAd | Different DNAe | ||
| R. speratusb | RSN-TKY | 1 | 18 | 0 | 3 | 0 | 4 | 26 | 13 | 23 |
| C. formosanus | CFN | 0 | 23 | 0 | 1 | 0 | 0 | 24 | 11 | 15 |
| N. koshunensis | NKN | 3 | 5 | 10 | 4 | 0 | 1 | 23 | 12 | 20 |
| C. domesticus | CDN | 0 | 8 | 10 | 4 | 0 | 1 | 23 | 15 | 21 |
| G. fuscus | GFN | 0 | 12 | 2 | 6 | 4 | 0 | 24 | 14 | 14 |
| H. sjoestedti | HSN | 0 | 1 | 22 | 0 | 0 | 1 | 24 | 6 | 15 |
| N. takasagoensis | NTN | 0 | 11 | 0 | 12 | 0 | 1 | 24 | 15 | 17 |
| O. formosanus | OFN | 0 | 0 | 1 | 23 | 0 | 0 | 24 | 7 | 7 |
| P. nitobei | PNN | 0 | 2 | 0 | 20 | 0 | 0 | 22 | 13 | 16 |
Termite origins of the isolated clones were clarified with these clone name tags.
Numbers of clones derived from R. speratus are cited from reference 28 and are restricted to those amplified with the primer pair of IGK and YAA.
As in the previous report on the analysis of nifH sequences in R. speratus (28), clones having significant similarity to nifH but with frameshift-like mutations, showing no translation of the nifH protein, were isolated from the four termites.
Number of clones with different amino acid (AA) sequences found in a single termite species.
Number of clones with different DNA sequences found in a single termite species.