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. 2022 May 30;17:2459–2474. doi: 10.2147/IJN.S363777

Figure 2.

Figure 2

Characteristics of peptide hydrogel and co-culture with hUC-MSCs. (A) Microstructure of peptide hydrogel including RADA16-I (i), RGD (ii), KLT (iii), and mixed peptide hydrogel (iv) were observed by atomic force microscopy. (B) Macrostructure of a self-assembled mixed peptide hydrogel using a transwell chamber (Corning 7007). Scale bar: 10 mm (i) Microstructure of a mixed peptide hydrogel was observed using scanning electron microscopy. Scale bar: 10 μm (ii) and 1 μm (iii). (C and D) The elemental composition of the mixed peptide hydrogel and their percentages were obtained by mapping analysis. (E) The state of the mixed peptide hydrogel co-cultured with hUC-MSCs in hUC-MSCs and hUC-MSCs+hydrogel groups was examined by AO/EB staining after 24, 48, and 72h. (F) The concentration of VEGF in the total protein and supernatant after co-culture of hUC-MSCs with or without mixed peptide hydrogels was measured with a VEGF kit. Scale bar: 100 µm. *p<0.05, ***p<0.001.