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. 1999 Nov;65(11):4935–4942. doi: 10.1128/aem.65.11.4935-4942.1999

TABLE 1.

Number of nifH clones obtained from RT-PCR products

Clone Affiliationa Most similar sequenceb (% amino acid identity) T-RF size No. of clonesc Accession no.
NKN-RT2-7 anf-methano I NKN9 (99.3) 54 6 AB027744
NKN-RT23 anf-methano I NKN9 (100) 54 5 AB027747
NKN-RT2-2 anf-methano I NKN9 (100) 54 1 AB027750
NKN-RT2-8 anf-methano I NKN9 (100) 54 1 AB027745
NKN-RT11 anf-methano I NKN9 (100) 161 4 AB027742
NKN-RT26 anf-methano I NKN9 (100) 161 1 AB027746
NKN-RT6 Anaerobe III NKN9 (97.9) 158 1 AB027749
NKN-RT16 Anaerobe III CDN28 (99.3) 132 1 AB027743
NKN-RT27 Pseudo nif I NKN24 (100) 43 1 AB027748
a

Roman numerals represent the clusters, which have been defined in reference 26. 

b

Clones NKN-RT23, -RT2-2, -RT2-8, -RT11, -RT26, and NKN9 (26) encoded an identical nifH amino acid sequence. 

c

Indicates the number of the clones among the 21 clones investigated showing an identical RFLP profile.