Fig. 2. Abundances of LHCSR1, LHCSR3.1, and PSBS1 transcripts after 1 hour in LL and HL, in the absence and presence of DCMU.

WT CC-125, grown as described in the legend of Fig. 1, was exposed to either white LL (30 μmol of photons m⁻² s⁻¹) or HL (480 μmol of photons m⁻² s⁻¹) for 1 hour, in the absence or presence of 10 μM DCMU, which was added to the cultures immediately before light exposure. Data were normalized to 1 (shown as dashed line in graph), which was set as the initial dark level of the mRNA. There was no significant difference of transcript levels comparing the same samples treated with and without DCMU (although slight differences are seen in this graph). Accumulation of PSBS1 transcripts between LL and HL was highly significant (P ≤ 0.0001), as well as the LHCSR1 transcript in cells treated with DCMU in LL and HL (P = 0.0153). n = 3 + SD. There are no statistical differences for transcript accumulations within the same light treatment comparing different DCMU treatments. Error bars represent +SD, n ≥ 3. One-way analysis of variance (ANOVA) was performed. *P < 0.05 and ****P < 0.0001. See data S1 for all statistical analysis.