Table 6.
Tumor growth-associated proteins in cyst fluid from cystic glioblastomas
| Protein | Function in tumor biology | N | Mean ± SD |
|---|---|---|---|
| SPARCL 1 | Glioma cell proliferation and invasion | 12 | 9.0 ± 0.4 |
| IGF-BP 2 | Glioma cell invasion. Vasculogenic mimicry | 12 | 8.3 ± 0.6 |
| Kininogen-1/bradykinin | Glioma cell migration. Endothelial leakage | 12 | 9.6 ± 0.2 |
| Kallikrein | Endothelial leakage. Anti-apoptotic | 12 | 7.9 ± 0.5 |
| Osteopontin | Tumor cell migration | 12 | 9.9 ± 0.5 |
| FAM3C | Invasion. Proliferation | 11 | 7.9 ± 0.3 |
| TREM2 | Glioma cell invasion. Proliferation | 9 | 7.8 ± 0.3 |
| CD166 (secreted) | Glioma cell invasion | 9 | 7.7 ± 0.5 |
| Prosaposin | Invasion. Anti-apoptotic | 7 | 7.5 ± 0.4 |
| Clusterin (secreted) | Anti-apoptotic | 12 | 10.2 ± 0.3 |
| Soluble CD5L | Anti-apoptotic | 10 | 8.1 ± 0.5 |
| HYOU1 | Anti-apoptotic. Hypoxia adaptation | 8 | 7.7 ± 0.6 |
| HGFA | Activates HGF | 9 | 7.7 ± 0.4 |
| HRG | Tumor vascularization | 12 | 10.1 ± 0.5 |
| Alpha-2-HS-glycoprotein | Tumor vascularization | 12 | 10.1 ± 0.5 |
| LRG | Tumor vascularization | 12 | 9.9 ± 0.4 |
| GRP78 (secreted) | Tumor vascularization | 12 | 8.6 ± 0.7 |
| MIF | Tumor vascularization | 9 | 7.9 ± 0.6 |
| VEGFA | Tumor vascularization. Vasculogenic mimicry. Endothelial leakage | 8 | 7.7 ± 0.2 |
| HDGF | Tumor vascularization | 2 | 7.8 ± 0.4 |
| Cadherin-5 (soluble) | Endothelial leakage. Vasculogenic mimicry | 12 | 8.2 ± 0.8 |
Cyst fluid from 12 cystic glioblastomas was sampled during tumor surgery and the proteome was analyzed by tandem mass spectrometry. Column “N” gives the number of samples out of 12 with a positive protein identification. Only samples with a positive protein identification were used to calculate mean ± SD values. Protein levels are given as log-10 of the area under the curve as determined by mass spectrometry. Abbreviations: CD5L: CD5-like protein (CD: cluster of differentiation); FAM3C: family with sequence similarity 3; FGF: fibroblast growth factor; GRP78: glucose-regulated protein 78; HDGF: Hepatoma-derived growth factor; HGF: Hepatocyte growth factor; HGFA: Hepatocyte growth factor activator; HRG: histidine-rich glycoprotein; HYOU1: hypoxia up-regulated protein 1; LRG: Leucine-rich α-2-glycoprotein; MIF: macrophage migration inhibitory factor; SPARCL1: secreted, acidic and rich in cysteine-like 1; TREM2: triggering receptor expressed on myeloid cells 2; VEGFA: vascular endothelial growth factor A