Fig. 2.

Circ_0004087 promotes the docetaxel resistance of PCa cells. (A) Schematic representation of the sites targeted by shRNAs designed for circ_0004087. (B) The levels of circ_0004087 and CDYL2 in the indicated stable cell lines. (C) The apoptosis rate of PCa cells expressing different circ_0004087 levels measured by flow cytometry. Cells were treated with 5 nM DTX for 48 h in this experiment. Cells went through the early apoptosis were marked with Annexin V-PE⁺/7-AAD⁻, cells went through the late apoptosis were marked with Annexin V-PE⁺/7-AAD⁺. (D) Fluorescent images of xenografts in nude mice acquired by an in vivo optical imaging system. (E) Images of excised xenografts acquired using the digital single-lens reflex camera. (F) Tumor volumes were measured every third day. (G) Tumor weights were measured on the day 30. (H) Immunohistochemical images of TUNEL staining on xenografts from each group 400 × . Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001