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. 2022 Jun 3;41:194. doi: 10.1186/s13046-022-02404-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Circ_0004087 interacts with SND1 in PCa cells. (A) Silver staining showing proteins that interact with circ_0004087 in PCa cells. Red boxes show the location of differential bands. (B) The flow chart for screening out the protein partner of circ_0004087. (C) Western blot verifying the protein pulled down by biotin-labeled circ_0004087 probe. (D) RNA-FISH and immunofluorescence staining assays exhibiting the subcellular localization of circ_0004087 (red) and SND1 (green) in PCa cell lines. Nuclei were stained with DAPI 1600 × . (E) The result of RIP assay with anti-SND1 antibody showed by agarose gel electrophoresis diagram. IgG was used as a negative control. (F) Quantification of the result of RIP assay with anti-SND1 antibody. IgG was used as a negative control. (G) The binding propensity between circ_0004087 and SND1 protein predicted by the catRAPID algorithm. (H) Structure diagrams showing full-length and truncated Flag-tagged recombinant SND1 proteins. (I) Western blot validating the full-length and truncated Flag-tagged recombinant SND1 proteins. (J) RIP assay with anti-Flag antibody revealing the binding domains of SND1 to circ_0004087. IgG was used as a negative control. Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001