Fig. 7.

Circ_0004087 regulates docetaxel resistance of PCa cells by mediating BUB1 expression. (A) Correlation analysis for circ_0004087 and BUB1 mRNA levels in PCa samples. (B) Protein levels of BUB1 in circ_0004087-overexpressing or knocking-down PCa cell lines. α-Tubulin was used as an internal control. (C-D) The apoptosis rate of PCa cells as indicated. Cells were treated with 5 nM DTX for 48 h. Cells went through the early apoptosis were marked with Annexin V-PE + /7-AAD-, cells went through the late apoptosis were marked with Annexin V-PE + /7-AAD + . (E) Images of excised xenografts acquired using the digital single-lens reflex camera. (F) Tumor weights were measured on the day 30. (G) Immunohistochemical images of TUNEL staining on xenografts from each group 400 × . Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001