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. 2022 Jun 3;13:227. doi: 10.1186/s13287-022-02903-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Ablation of FGFR3⁺ cells inhibits the postnatal development of NP tissue. (a) Schema shows the depletion of the FGFR3⁺ cells by Tam administration in FGFR3-CreERT2;Rosa26-DTA (R3;DTA) mice. (b) Representative Safranin O/Fast green staining images reveal pathological changes in IVD from R3;DTA mice induced with Tam at P7 and sacrificed at 2w. Scale bars, 100 µm. (c) Height, area and cell counting of NP of R3;DTA mice compared with the control FGFR3-CreERT2 after Tam administration are compared, respectively. ****P < 0.0001, Unpaired Student’s t test. (d) TUNEL assay and Ki67 immunostaining were performed to detect the changes of death and proliferation of NP (dotted circle) cells after FGFR3P^·+ cell depletion. Numbers indicate the percentages of TUNEL and Ki67 positive cells over total NP cells. Scale bars, 100 µm