Table 2.
Absolute recovery, matrix effects and normalized matrix effects of ( ±)-primaquine, ( ±)-carboxyprimaquine and their isotope-labelled internal standard in human EDTA plasma
| Analyte | Sample | R1 | R2 | R3 | R4 | R5 | Average | SD | CV (%) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Absolute recovery (%), (n = 5) | ||||||||||
| ( +)-PRQ | QC1 | 75.3 | 73.3 | 76.7 | 74.6 | 71.3 | 74.2 | 2.05 | 2.76 | |
| QC3 | 72.3 | 78.4 | 72.3 | 70.9 | 70.5 | 72.9 | 3.19 | 4.38 | ||
| IS QC1 | 76.8 | 76.4 | 76.1 | 71.9 | 73.2 | 74.9 | 2.19 | 2.92 | ||
| IS QC3 | 74.0 | 78.4 | 73.7 | 72.5 | 71.1 | 73.9 | 2.74 | 3.71 | ||
| (−)-PRQ | QC1 | 78.1 | 72.3 | 73.1 | 71.0 | 75.3 | 74.0 | 2.79 | 3.78 | |
| QC3 | 76.8 | 70.7 | 75.7 | 74.9 | 72.3 | 74.1 | 2.51 | 3.39 | ||
| IS QC1 | 73.3 | 73.4 | 73.3 | 75.3 | 70.5 | 73.2 | 1.71 | 2.34 | ||
| IS QC3 | 78.2 | 71.4 | 75.9 | 75.3 | 73.6 | 74.9 | 2.55 | 3.40 | ||
| ( +)-CPRQ | QC1 | 75.1 | 75.1 | 79.2 | 73.0 | 72.3 | 74.9 | 2.69 | 3.59 | |
| QC3 | 75.2 | 75.2 | 78.0 | 74.7 | 75.4 | 75.7 | 1.31 | 1.73 | ||
| IS QC1 | 77.6 | 77.6 | 80.1 | 75.2 | 77.8 | 77.7 | 1.73 | 2.23 | ||
| IS QC3 | 73.5 | 73.5 | 74.6 | 72.9 | 73.1 | 73.5 | 0.66 | 0.89 | ||
| (−)-CPRQ | QC1 | 79.2 | 79.8 | 74.1 | 76.8 | 70.5 | 76.1 | 3.85 | 5.05 | |
| QC3 | 76.5 | 78.9 | 75.5 | 76.7 | 73.4 | 76.2 | 2.00 | 2.62 | ||
| IS QC1 | 73.7 | 76.3 | 70.4 | 72.9 | 73.2 | 73.3 | 2.11 | 2.87 | ||
| IS QC3 | 75.4 | 78.2 | 74.6 | 75.2 | 73.8 | 75.4 | 1.66 | 2.21 | ||
| Analyte | Sample | R1 | R2 | R3 | R4 | R5 | R | Average | SD | CV (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| Matrix effects, (n = 6) | ||||||||||
| ( +)-PRQ | QC1 | 1.08 | 1.05 | 1.14 | 1.15 | 1.20 | 1.03 | 1.11 | 0.07 | 5.91 |
| QC3 | 1.01 | 0.91 | 1.03 | 1.00 | 0.96 | 1.12 | 1.01 | 0.07 | 7.03 | |
| IS QC1 | 0.98 | 0.97 | 0.98 | 1.13 | 1.04 | 1.00 | 1.02 | 0.06 | 5.99 | |
| IS QC3 | 0.91 | 1.04 | 0.93 | 0.94 | 0.85 | 0.97 | 0.94 | 0.06 | 6.73 | |
| (−)-PRQ | QC1 | 1.14 | 1.10 | 1.08 | 1.15 | 1.14 | 1.09 | 1.12 | 0.03 | 2.70 |
| QC3 | 0.95 | 1.08 | 1.06 | 1.20 | 1.06 | 1.04 | 1.07 | 0.08 | 7.55 | |
| IS QC1 | 1.09 | 1.11 | 1.09 | 1.07 | 0.98 | 1.10 | 1.07 | 0.05 | 4.44 | |
| IS QC3 | 1.06 | 1.01 | 1.02 | 1.09 | 1.12 | 1.02 | 1.05 | 0.04 | 4.23 | |
| ( +)-CPRQ | QC1 | 0.83 | 0.89 | 1.03 | 1.06 | 0.92 | 0.96 | 0.95 | 0.09 | 9.13 |
| QC3 | 1.10 | 1.20 | 1.17 | 1.08 | 1.04 | 1.06 | 1.11 | 0.06 | 5.72 | |
| IS QC1 | 0.94 | 1.10 | 1.05 | 0.94 | 0.92 | 0.98 | 0.99 | 0.07 | 7.33 | |
| IS QC3 | 1.06 | 1.14 | 1.22 | 1.12 | 1.04 | 1.17 | 1.13 | 0.07 | 6.00 | |
| (−)-CPRQ | QC1 | 0.99 | 1.02 | 1.09 | 0.93 | 0.95 | 1.04 | 1.00 | 0.06 | 5.90 |
| QC3 | 1.10 | 1.15 | 1.14 | 1.03 | 1.08 | 1.06 | 1.09 | 0.05 | 4.24 | |
| IS QC1 | 1.08 | 1.09 | 1.08 | 1.09 | 1.02 | 1.03 | 1.07 | 0.03 | 2.95 | |
| IS QC3 | 1.02 | 1.19 | 1.05 | 0.99 | 0.99 | 1.01 | 1.04 | 0.08 | 7.30 | |
| Normalised matrix effects, (n = 6) | ||||||||||
| ( +)-PRQ | QC1/IS QC1 | 1.10 | 1.08 | 1.16 | 1.02 | 1.15 | 1.03 | 1.09 | 0.06 | 5.57 |
| QC3/IS QC3 | 1.11 | 0.88 | 1.11 | 1.06 | 1.13 | 1.15 | 1.07 | 0.10 | 9.47 | |
| (−)-PRQ | QC1/IS QC1 | 1.05 | 0.99 | 0.99 | 1.07 | 1.16 | 0.99 | 1.04 | 0.07 | 6.59 |
| QC3/IS QC3 | 0.90 | 1.07 | 1.04 | 1.10 | 0.95 | 1.02 | 1.01 | 0.08 | 7.62 | |
| ( +)-CPRQ | QC1/IS QC1 | 0.88 | 0.81 | 0.98 | 1.13 | 1.00 | 0.98 | 0.96 | 0.11 | 11.5 |
| QC3/IS QC3 | 1.04 | 1.05 | 0.96 | 0.96 | 1.00 | 0.91 | 0.99 | 0.05 | 5.54 | |
| (−)-CPRQ | QC1/IS QC1 | 0.92 | 0.94 | 1.01 | 0.85 | 0.93 | 1.01 | 0.94 | 0.06 | 6.33 |
| QC3/IS QC3 | 1.08 | 0.97 | 1.09 | 1.04 | 1.09 | 1.05 | 1.05 | 0.05 | 4.42 | |
CPRQ, carboxyprimaquine; CV, coefficient of variation; IS, Internal standard; PRQ, primaquine; QC, quality control; R, Replicate; SD, standard deviation