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. 2022 Jun 3;13:223. doi: 10.1186/s13287-022-02879-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2. — 253G1 hiPSCs on iMatrix-511 and GNF with ON2 medium maintained normal karyotype and differentiation ability. a Normal karyotypes of 253G1 hiPSCs maintained on both iMatrix-511 and GNF over 20 passages with ON2 were identified by G-band karyotype analysis (46 XX). b All three germ layer differentiation in vitro of 253G1 hiPSCs occurred after expansion on iMatrix-511 or GNF with ON2. Ectodermal, mesodermal, and endodermal cell types were revealed by specific markers: β-tubulin III, myosin, and AFP, respectively, by immunocytochemistry. Scale bar, 100 µm. c Histological analysis of teratomas generated in SCID mice from 253G1 iPSCs after ON2 culture on iMatrix-511 or GNF, respectively. Neuro-epithelium, cartilage, and gut-like epithelium were visualized by hematoxylin and eosin staining. Scale bar, 200 µm