Table 1.
Method validation for the precision (% RSD) and accuracy (% bias) of the UHPLC-MS/MS method for the determination of remdesivir in the maternal blood, placenta, fetus, and amniotic fluid dialysate of pregnant rats.
| Nominal Concentration (ng/mL) | Intra-day |
(n=5) |
Inter-day |
(n=3) |
||
|---|---|---|---|---|---|---|
| Observed Concentration (ng/mL) | Precision (% RSD) | Accuracy (% bias) | Observed concentration (ng/mL) | Precision (% RSD) | Accuracy (% bias) | |
| Maternal blood | ||||||
| 0.5 | 0.49 ± 0.08 | 16.30 | -1.82 | 0.55 ± 0.06 | 10.40 | 9.33 |
| 1 | 1.08 ± 0.05 | 4.28 | 7.80 | 1.10 ± 0.14 | 12.97 | 9.67 |
| 10 | 11.03 ± 0.56 | 5.06 | 10.28 | 10.03 ± 0.58 | 5.80 | 0.33 |
| 100 | 103.52 ± 4.58 | 4.42 | 3.52 | 100.65 ± 0.85 | 0.84 | 0.65 |
| Placenta | ||||||
| 0.5 | 0.47 ± 0.02 | 4.45 | -6.27 | 0.56 ± 0.08 | 13.32 | 12.67 |
| 1 | 0.99 ± 0.04 | 3.98 | -1.22 | 0.97 ± 0.12 | 12.77 | -3.33 |
| 10 | 10.03 ± 0.42 | 4.15 | 0.25 | 9.75 ± 0.32 | 3.28 | -2.47 |
| 100 | 100.12 ± 2.00 | 1.99 | 0.12 | 99.89 ± 0.70 | 0.70 | -0.11 |
| Fetus | ||||||
| 0.5 | 0.57 ± 0.02 | 3.82 | 14.35 | 0.50 ± 0.06 | 11.62 | -0.67 |
| 1 | 1.06 ± 0.06 | 5.38 | 5.64 | 1.08 ± 0.13 | 11.61 | 7.67 |
| 10 | 9.76 ± 0.23 | 2.33 | -2.38 | 10.16 ± 0.28 | 2.79 | 1.57 |
| 100 | 100.78 ± 2.06 | 2.04 | 0.78 | 99.95 ± 0.16 | 0.16 | -0.05 |
| Amniotic fluid | ||||||
| 0.5 | 0.57 ± 0.04 | 7.20 | 14.02 | 0.50 ± 0.05 | 9.93 | -0.67 |
| 1 | 1.11 ± 0.01 | 1.14 | 10.73 | 1.02 ± 0.09 | 8.71 | 2.00 |
| 10 | 9.80 ± 0.61 | 6.24 | -1.99 | 10.53 ± 0.30 | 2.86 | 5.30 |
| 100 | 100.49 ± 1.03 | 1.02 | 0.49 | 99.98 ± 0.04 | 0.04 | - 0.02 |
Data have been expressed as mean ± SD.