Table 2.
Method validation for the precision (% RSD) and accuracy (% bias) of the UHPLC-MS/MS method for determination of GS-441524 in the maternal blood, placenta, fetus and amniotic fluid dialysate of pregnant rats.
| Nominal concentration (ng/mL) | Intra-day |
(n=5) |
Inter-day |
(n=3) |
||
|---|---|---|---|---|---|---|
| Observed concentration (ng/mL) | Precision (% RSD) | Accuracy (% bias) | Observed concentration (ng/mL) | Precision (% RSD) | Accuracy (% bias) | |
| Maternal blood | ||||||
| 5 | 5.04 ± 0.42 | 8.32 | 0.77 | 4.82 ± 0.13 | 2.76 | -3.53 |
| 10 | 10.10 ± 0.65 | 6.42 | 0.98 | 10.04 ± 0.28 | 2.78 | 0.40 |
| 100 | 101.90 ± 4.39 | 4.30 | 1.90 | 96.76 ± 6.04 | 6.24 | -3.24 |
| 500 | 501.3 ± 26.26 | 5.24 | 0.26 | 499.01 ± 3.50 | 0.70 | -0.20 |
| Placenta | ||||||
| 5 | 5.07 ± 0.69 | 13.60 | 1.33 | 5.39 ± 0.87 | 16.20 | 7.87 |
| 10 | 10.14 ± 0.67 | 6.61 | 1.39 | 10.65 ± 0.75 | 7.00 | 6.47 |
| 100 | 101.80 ± 4.09 | 4.01 | 1.80 | 99.95 ± 3.61 | 3.62 | -0.05 |
| 500 | 504.17 ± 4.70 | 0.93 | 0.83 | 501.25 ± 1.37 | 0.27 | 0.25 |
| Fetus | ||||||
| 5 | 5.76 ± 0.61 | 10.46 | 15.80 | 5.25 ± 0.15 | 2.86 | 5.00 |
| 10 | 10.39 ± 1.13 | 10.92 | 3.89 | 10.67 ± 1.16 | 10.87 | 6.67 |
| 100 | 103.17 ± 6.57 | 6.37 | 3.17 | 100.35 ± 2.23 | 2.22 | 0.35 |
| 500 | 504.01 ± 4.51 | 0.90 | 0.80 | 504.90 ± 7.51 | 1.49 | 0.98 |
| Amniotic fluid | ||||||
| 5 | 5.13 ± 0.27 | 5.17 | 2.57 | 5.29 ± 0.19 | 3.66 | 5.87 |
| 10 | 10.43 ± 0.92 | 8.82 | 4.34 | 10.60 ± 0.57 | 5.38 | 5.97 |
| 100 | 105.0 ± 2.98 | 2.84 | 5.00 | 98.28 ± 9.78 | 9.95 | -1.72 |
| 500 | 500.98 ± 1.49 | 0.30 | 0.20 | 500.45 ± 1.47 | 0.29 | 0.09 |
Data have been expressed as mean ± SD.