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. 2022 Jun 3;13:3101. doi: 10.1038/s41467-022-29949-3

Fig. 2. PBMC response to Cbeg5.

Fig. 2

Fresh PBMC were isolated from healthy patients and exposed to Cbeg5, Fn5 and PBS for 6 h and analyzed by CyTOF with a panel of 16 cell surface and 10 cytokine markers. a Cell surface markers distinguish major immune cell populations (A–K) that correlate to groups identified by tSNE analysis (Supplementary Fig. 2). b Fold induction of 10 cytokines across cell populations A–K from (a). Fold induction is calculated relative to PBS control. Cytokine responses > 3-fold to > 100-fold are marked with gray-shaded boxes. Proteins assayed at 100 nM or 1000 nM as indicated. (Data is a single experiment. PBS data is normalized to a replicate experiment with PBS). c Bar graph (mean ± s.e.m) of percent positive cells for each cytokine in specific cell populations in response to Cbeg5 or FN3 control at 500 nM concentration (N = 5 from three independent experiments, unpaired, two-sided Mann–Whitney test comparing Cbeg5 to Fn3 control in each cell population) ***p < 0.001 **p < 0.01 *p < 0.05.