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. 2022 Jun 3;13:3101. doi: 10.1038/s41467-022-29949-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology (a) and for change in immune cell populations by flow cytometry (b, c). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. 5). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII⁺CD11c⁺CD64⁺CD11b⁺ and monocytes MHCII^-Lyc⁺CD11b⁺. c There was a significant increase in lamina propria MHCII⁺CD11c⁺CD64⁺CD11b⁺ macrophages and a decrease in MHCII⁻Lyc⁺CD11b⁺ monocytes in the treatment group (N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).