Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 3;13:3102. doi: 10.1038/s41467-022-30776-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Heatmap showing cell type specific mRNA abundance of the 6 DA-FASS proteins selected for further experimental validation (detailed from Fig. 3h). b–g Epifluorescence images of a representative sample of synaptosome populations labelled with b–d, f, g anti-Th (green) or e DAT and b anti-Cpne7, c Mint-1/Apba1, d Cadps2, e SynCAM 2/Cadm2, f Stx4, g Mgll (magenta) and analysis of staining showing particle proportions per frame. All data are mean ± SEM and pulled from N = 2 to N = 3 independent sorts and n = 5 to n = 11 field of view per independent sort. Each independent sort pooled at least 3 animals. Statistical significance was analyzed using Two-way ANOVA, b Th/Cpne7: Interaction F_2,180 = 131.9 ****p < 0.0001, Condition F_1,180 = 0.0004 p = 0.984, Immunolabelling F_2,180 = 570.4 ****p < 0.0001; c Th/Mint-1: Interaction F_2,183 = 163.7 ****p < 0.0001, Condition F_1,183 = 0.0009 p = 0.975, Immunolabelling F_2,183 = 316.5 ****p < 0.0001; d Th/Cadps2: Interaction F_2,187 = 110.5 ****p < 0.0001, Condition F_1,187 = 0.004 p = 0.951, Immunolabelling F_2,187 = 88.12 ****p < 0.0001; e DAT/SynCAM 2: Interaction F_2,60 = 84.92 ****p < 0.0001, Condition F_1,60 = 4.371e-005 p = 0.995, Immunolabelling F_2,60 = 73.75 ****p < 0.0001 f Th/Stx4: Interaction F_2,177 = 69.25 ****p < 0.0001, Condition F_1,177 = 0.004 p = 0.95, Immunolabelling F_2,177 = 49.15 ****p < 0.0001; g Th/Mgll: Interaction F_2,192 = 98.57 ****p < 0.0001, Condition F_1,192 = 4.217e-005 p = 0.995, Immunolabelling F_2,192 = 1242 ****p < 0.0001 with Šídák’s multiple comparisons test. For all panels, scale bar = 1 μm. h Correlation between protein immunodetection and label-free mass spectrometry-based enrichment ratios (Two-tailed Pearson’s correlation coefficient *p = 0.021, r² = 0.62). Correlated data are pulled from independent experiments. Dot sizes are scaled to the proportion of dopaminergic synaptosomes expressing each marker.