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. 2022 Jun 3;13:3102. doi: 10.1038/s41467-022-30776-9

Fig. 5. Proteomics and immunofluorescence of DA-FASS sample reveals dopamine synapse association with other synaptic partners.

Fig. 5

a Scheme of the molecular organization of the synaptic vesicle cycle, c glutamatergic, e GABAergic and g cholinergic synapses (Adapted from the database KEGG). Proteins enriched in DA-FASS samples are in red, depleted in cyan, retained in grey, and absent in white. Gene names for each protein can be found in Supplementary table 4 (ST4). b, d, f, h Epifluorescence images of a representative sample of synaptosome populations before and after sorting labelled with anti-EGFP or anti-Th (green) and b anti-Synapsin, d VGLUT1, f VIAAT and h VAChT (magenta). Quantification of stainings showing particle proportions per frame. All data are mean ± SEM and pulled from N = 2 to N = 3 independent sorts and n = 4 to n = 11 field of view per independent sort. Each independent sort pooled at least 3 animals. b EGFP/Synapsin: Interaction F2,51 = 237,8 ****p < 0.0001, Condition F1,51 = 0.01 p = 0.92, Immunolabelling F2,51 = 237.5 ****p < 0.0001. d EGFP/VGLUT1 Interaction F2,63 = 91.49 ****p < 0.0001, Condition F1,63 = 0.002 p = 0.97, Immunolabelling F2,63 = 92.06 ****p < 0.0001. f EGFP/VIAAT: Interaction F2,78 = 54.90 ****p < 0.0001, Condition F1,78 = 0.04 p = 0.844, Immunolabelling F2,78 = 55.34 ****p < 0.0001. h Th/VAChT Interaction F2,144 = 180.3 ****p < 0.0001, Condition F1,144 = 0.016 p = 0.9, Immunolabelling F2,144 = 412.2 ****p < 0.0001 with Šídák’s multiple comparisons test. For all panels, scale bar = 1 μm. See VGLUT2 immunofluorescence analysis in Supplementary Fig. 4.