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. 2022 Jun 3;13(6):523. doi: 10.1038/s41419-022-04980-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Determination of the SGLT2 mRNA expression level by the GEPIA web tool. Boxplot analysis of the expression level showing log2 (TPM + 1) on a log-scale. RT-PCR analysis (b) and Western blot analysis (c) of the mRNA and protein expression of SGLT2 in eight paired osteosarcoma tumor tissues (T) and the matched adjacent normal tissues (ANT) of the same patient. GAPDH served as an internal reference. d RT-PCR analysis of the mRNA expression of SGLT2 in a normal osteogenic cell line (hFOB1.19 cell line) and four osteosarcoma cell lines (MG-63, U2OS, MNNG/HOS, and 143B cell lines). GAPDH served as an internal reference. e Western blot analysis of the protein expression of SGLT2 in a normal osteogenic cell line (hFOB1.19 cell line) and four osteosarcoma cell lines (MG-63, U2OS, MNNG/HOS, and 143B cell lines). Histogram to show the relative protein level of SGLT2 (n = 3). GAPDH served as an internal reference. f IHC images of SGLT2-stained Tissue Microarrays (TMAs). Scale bars are shown in the figure. g Dot plots of the IHC score of SGLT2 expression of TMA tissue sections (normal osteogenic specimens: n = 19, osteosarcoma specimens: n = 69, p < 0.001). Statistical analyses were performed using the D’Agostino and Pearson omnibus normality test.