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. 2022 Jun 3;13(6):523. doi: 10.1038/s41419-022-04980-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — LC-MS/MS analysis of the interaction between SGLT2 and TRIM21 (a) based on the detection of the peptide of TRIM21 (b). c Analysis of the interaction between SGLT2 and TRIM21 by co-immunoprecipitation. Western blot analysis of the protein expression levels of SGLT2 in TRIM21-overexpressed (d) and -silenced (e) MG-63 and MNNG/HOS cells. GAPDH served as an internal reference. f Western blot analysis in MG-63 cells transfected with sh-TRIM21 or TRIM21 plasmid for 48 h. Cells were treated with MG132 for 8 h before harvesting. g Western blot analysis of the protein expression levels of SGLT2 in TRIM21-overexpressed MG-63 and MNNG/HOS cells for 48 h. Cells were treated with MG132 for 8 h before harvesting. GAPDH served as an internal reference.