Figure 5.
PTS attenuates H2O2-induced cardiomyocyte apoptosis depending on the regulation of Nrf2 activity. ML385, a direct activator of the Keap1 and Nrf2 interaction, was used to cotreat H9c2 with vehicle or PTS. (a) Western blotting results showed the changes in apoptosis-related proteins in H9c2 cells after Nrf-2 blockade. (b–e) The levels of cleaved caspase-3, cleaved PRAR-1, Bax, and Cyt-c were quantified using ImageJ. Data are expressed as the mean ± SEM (n = 3). ∗P < 0.05, ∗∗P < 0.01; ns: not significant.