Fig. 2.

IFB-088 treatment improves MpzR98C/ + mice quadriceps femoral nerve morphology. a g-ratios performed on toluidine blue–stained semi-thin sections of quadriceps femoral nerve from MpzR98C/ + mice treated with vehicle b.i.d. or IFB-088 1 mg/kg b.i.d. for 5 months. Data are expressed as mean ± SEM. n = 4 mice per condition. *P < 0.05 by Student’s t-test. b Scatter plot of quadriceps femoral nerve g-ratios toward the axon diameter from MpzR98C/ + mice treated with vehicle b.i.d. or IFB-088 1 mg/kg b.i.d. n = 4 mice per condition. c Average g-ratio plotted by axon diameter. *P < 0.05 by Student’s t-test. d Myelin thickness measured from toluidine blue–stained semi-thin sections of quadriceps femoral nerve from MpzR98C/ + mice treated with vehicle b.i.d. or IFB-088 1 mg/kg b.i.d. for 5 months. Data are expressed in µm as mean ± SEM. n = 4 mice per condition. *P < 0.05 by Student’s t-test. e Scatter plot of quadriceps femoral nerve myelin thickness toward axon diameter from MpzR98C/ + mice treated with vehicle b.i.d. or IFB-088 1 mg/kg b.i.d. n = 4 mice per condition. f Average myelin thickness plotted by axon diameter. *P < 0.05 by Student’s t-test. g Ultrathin electron microscopy sections of quadriceps femoral nerve from WT mice treated with vehicle b.i.d. and from MpzR98C/ + mice treated with vehicle b.i.d. or IFB-088 1 mg/kg b.i.d. for 5 months. Lower panels are higher magnification images of upper panels. Scale bar 1 µm for top panels, and 500 nm for bottom panels. The insets show normal myelin compaction and periodicity