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. 2022 Jun 4;12:9316. doi: 10.1038/s41598-022-13417-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intendhttp://creativecommons.org/licenses/by/4.0/ed use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit .

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Sample case for use of plasma circulating tumor DNA to detect HNSCC (Case 10). Clinical course of patient #10. This patient exhibited a p16-negative oropharyngeal carcinoma cT3N0M0 and was treated with chemoradiotherapy. The patient was treated with a cisplatin combination. Before treatment, ctDNA was detected via PCLO/P516S. Post-treatment imaging studies showed that the tumor had disappeared, but ctDNA was still detected 6 months after treatment. Tumor recurrence was detected 2 years after treatment during follow-up imaging.