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Mutation rates of the APT gene using the CRISPR–Cas and TALEN systems.
| Editing strategy | Regenerant clones | 2-FAR clones | Relative mutation efficiency (%)a |
|---|---|---|---|
| CRISPR–Cas (sgRNA#1) | 71,100 | 1718 | 2.41 ± 0.17 b |
| CRISPR–Cas (sgRNA#2) | 61,600 | 2077 | 3.39 ± 0.25 |
| TALEN (TALEn402 + 405) | 68,300 | 56 | 0.08 ± 0.01 |
aRelative mutation efficiency expresses the frequency of 2-FA resistant clones among the population of regenerants.
bAverage and standard deviations were determined from three independent experiments.
