TABLE 4.
Concentrations of EPS and activity of FBPase of strain NZ9000 carrying pNZ4030 and pNZ4150 or pNZ8048 incubated in CDM with 0.5% fructose at 30°C and induced with different levels of nisin at an OD600 of 0.1
| Strain | Nisin (ng ml−1) | EPS (mg liter−1) | OD600 | EPS (mg OD unit−2) | μa (h−1) | FBPase (nmol mg of protein−1 min−1) |
|---|---|---|---|---|---|---|
| NZ9000 + pNZ4030 + pNZ8048 | 0 | 1.4 | 1.045 | 1.37 | 0.22 | 6.2 |
| NZ9000 + pNZ4030 + pNZ8048 | 1 | 1.6 | 1.115 | 1.37 | 0.27 | 6.3 |
| NZ9000 + pNZ4030 + pNZ4150 | 0 | 1.3 | 1.07 | 1.21 | 0.23 | 6.7 |
| NZ9000 + pNZ4030 + pNZ4150 | 0.001 | 1.5 | 1.12 | 1.34 | 0.27 | 11.4 |
| NZ9000 + pNZ4030 + pNZ4150 | 0.01 | 2.4 | 1.305 | 1.84 | 0.47 | 78 |
| NZ9000 + pNZ4030 + pNZ4150 | 0.1 | 5.4 | 1.63 | 3.31 | 0.60 | 303 |
| NZ9000 + pNZ4030 + pNZ4150 | 1 | 8.5 | 1.72 | 4.94 | 0.74 | 628 |
a
μ, maximum growth rate after induction. The initial growth rate for both strains was 0.47 h−1.