. 2022 Jun 2;10:e13524. doi: 10.7717/peerj.13524

Table 2. Kinetic analysis of Na⁺/K⁺-ATPase activity from the brain porcine in the absence or presence of cardenolides calotropin and C3OG at different concentrations.

Compound	Concentration (μM)	K_m (mM)	V_max (μmolPi/mg/min)	K_i (μM)	Type of inhibition
Calotropin	0.0	0.68 ± 0.12	3.56 ± 0.06	0.20 ± 0.03	Uncompetitive
	0.1	0.45 ± 0.07	2.17 ± 0.10
	1.0	0.17 ± 0.03	0.77 ± 0.10
C3OG^a	0.0	0.66 ± 0.1	2.29 ± 0.10	0.52 ± 0.05	Uncompetitive
	0.1	0.42 ± 0.04	1.47 ± 0.08
	1.0	0.12 ± 0.01	0.80 ± 0.06

Notes:

Corotoxigenin 3-O-glucopyranoside.

All values were obtained from triplicate independent assays. The data are expressed as mean ± standard deviation. The values of K_m and V_max were calculated by data fitting Michaelis-Menten non-linear regression. K_m is expressed in mM. V_max is expressed as μmol of Pi released/mg of protein/min.

Table 2. Kinetic analysis of Na+/K+-ATPase activity from the brain porcine in the absence or presence of cardenolides calotropin and C3OG at different concentrations.

Table 2. Kinetic analysis of Na⁺/K⁺-ATPase activity from the brain porcine in the absence or presence of cardenolides calotropin and C3OG at different concentrations.