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. 2022 May 30;20:2759–2777. doi: 10.1016/j.csbj.2022.05.052

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Differential expression analysis of poly-(A) enriched RNAs in TBEV-infected neurons and astrocytes reveals strain-specific and cell-specific responses. (A) Graphic summary of poly-(A) classes identified as differentially expressed upon TBEV infection. (B) Graphic summary of DE genes (protein-coding mRNAs and non-coding RNAs) in the respective dataset. (C) Heatmap visualizing the overall expression pattern of protein-coding mRNAs in analysed samples. (D) Heatmap visualizing the overall expression pattern of non-coding RNAs in analysed samples. (E) Verification of RNA-seq data by qRT-PCR. Relative expressions of HSPA-6, OASL, RNVU1, and RSAD2 (viperin) genes were processed via the ΔΔ-ct method with HPRT1 as a reference gene. All samples were analysed in biological and technical triplicates. Spearman correlation was evaluated with GraphPad Prism software. (F) Gene set enrichment analysis (DAVID tool; GO Biological processes; p < 0.001) of significantly up-regulated genes in analysed samples performed by DAVID tool. (G) Gene set enrichment analysis (DAVID tool; GO Biological processes; p < 0.001) of significantly down-regulated genes in analysed samples performed by DAVID tool.