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TMAO regulates the expression of DEGs related to patient outcome in TNF-α induced inflammatory HCC. (A–D) Western blotting was used to analyze the expression level of Periostin, Snab, Layilin and Htra3 in Hepa1-6 and Huh7 cells treated with 50 ng/ml TNF-α combined with 50 μM TMAO or 50 ng/ml TNF-α alone. Grayscale values were analyzed; data are mean ± SD (n = 3 per group); Student’s t-test; ***, p < 0.001.
