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. 2022 Apr 7;39(6):msac078. doi: 10.1093/molbev/msac078

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© The Author(s) 2022. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 4. — Gene function analysis of sh1 in foxtail millet. (a) Expression levels of sh1 in multiple organs including the root, stem, leaf, leaf sheath (LS), bristle, and panicles at different stages (Panicle1, before heading; Panicle2, 3 d after heading; Panicle3, 8 d after heading; Panicle4, grain-filling stage). (b) Subcellular localization of the SH1–GFP fusion protein in foxtail millet leaf cells. (c) Dual-luciferase transient activity assays determined that SH1 functioned as a transcriptional repressor. The GAL4DB–VP16–SH1 fusion protein dramatically (P = 6.9 × 10⁻⁵) repressed luciferase activity in comparison to the control protein GAL4DB–VP16. **, strongly significant; error bars, SD (n = 3).