Figure 6.

Liver mitochondrial activity correlates with metabolic phenotypes, and correlations between complexes are disrupted by HFD in males

(A) Citrate synthase activity (A.U.). All sexes and diets are pictured together as they differed only minorly, showing that CS is principally affected by strain. Boxplot lower and upper hinges correspond to the first and third quartiles, and center line is the median. The whiskers extend from the hinge to the largest value no further than 1.5 ∗ inter-quartile range.

(B) Complex II and complex III activity (normalized by citrate synthase activity) is affected by diet in sex-specific manner.

(C) Proportion of variance of liver mitochondrial activity explained by strain, sex, diet, sex-by-diet interactions (sex:diet), and citrate synthase (CS) activity. Each bar corresponds to the activity of the different complexes of the electron transport chain as well as the citrate synthase activity or a model that takes all the complexes together into account (ALL). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ANOVA test.

(D) Spearman’s correlations between the activity of liver mitochondrial complexes and essential cardio-metabolic traits. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, correlation test, corrected for multiple testing with the BH-FDR procedure.

(E) Correlation network of liver mitochondrial complex activities in each condition. The width of the edges corresponds to the magnitude of positive correlation between complexes. Only significant edges are shown (Benjamini-Hochberg adjusted-p <0.05).