. 2022 May 23;13:864674. doi: 10.3389/fimmu.2022.864674

Table 2.

Antigen specific geometric mean memory B cell frequencies per age group with 95% confidence intervals and significances from all countries together were calculated using a linear mixed model on the log10 transformed data taking the longitudinal structure into account.

Antigen	Timepoint	Children	Adolescents	Young adults	Older adults
Ptx GM Bmem frequencies /100,000 PBMCs (CI)	day 0	0.5 (0.4-0.8)	0.9 (0.6-1.3)^a,b	0.4 (0.3-0.6)^c	0.4 (0.3-0.6)^c
	day 28	3.4 (2.2-5.1)	4.9 (3.3-7.3)^a,b	2.5 (1.7-3.6)^c	2.0 (1.3-2.9)^c
	1 year	0.8 (0.6-1.3)	1.4 (1.0-2.1)	1.0 (0.7-1.5)	0.8 (0.5-1.2)
FHA GM Bmem frequencies /100,000 PBMCs (CI)	day 0	2.1 (1.5-3.0)^a,b	2.9 (2.1-4.0)^a,b	0.7 (0.5-1.0)^c,d	0.8 (0.6-1.0)^c,d
	day 28	18 (13-25)^b	21 (15-29)^b	19 (14-25)^b	8.7 (6.4-12)^a,c,d
	1 year	4.2 (3.0-5.8)^b	6.7 (4.9-9.3)^a,b	3.7 (2.7-5.0)^c	2.4 (1.7-3.3)^c,d
Prn GM Bmem frequencies /100,000 PBMCs (CI)	day 0	0.6 (0.4-0.9)^a,b	0.8 (0.5-1.1)^a,b	0.4 (0.3-0.5)^c,d	0.3 (0.2-0.5)^c,d
	day 28	8.1 (5.5-12)^b	13 (8.6-18)^b	9.2 (6.4-13)^b	3.0 (2.1-4.3)^a,c,d
	1 year	1.8 (1.2-2.6)^b	2.7 (1.9-4.0)^b	1.8 (1.3-2.7)^b	0.8 (0.6-1.2)^a,c,d

Significance per antigen has been tested between age groups within a timepoint; p ≤ 0·05. Significantly different from, ^ayoung adults; ^bolder adults; ^cadolescents; ^dchildren. Ptx, pertussis toxin; FHA, filamentous haemagglutinin; Prn, pertactin; GM, geometric mean; Bmem, memory B cell; PBMC, peripheral blood mononuclear cells; CI, 95% confidence interval.