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. 2022 Jun 4;3(2):101423. doi: 10.1016/j.xpro.2022.101423

Figure 2.

Figure 2

Analysis of amino acid sites involved in drug-target engagement between CypA and CsA

(A) Cellular thermal shift assay of WT CypA or mutated CypA in HEK 293T cells treated with or without CsA. The first lane shows the four samples without any heating. The Flag band and the Coomassie blue staining are used as loading controls.

(B) The relative immunoblotting band density is analyzed. All data are shown as the mean ± SD and are representative of three independent experiments. ∗∗∗p < 0.001.